INVESTIGATION OF ANTI DIABETIC ACTIVITY OF IPOMOEA RENIFORMIS

Abstract

Background  : Diabetes mellitus is a widespread metabolic disorder, urging the search for effective treatments. Ipomoea reniformis, a traditional medicinal plant, is gaining attention for its potential anti-diabetic properties due to its rich phytochemical composition. Combining in vitro experiments and computational analysis, this study aims to explore the mechanisms behind its effects, including cell viability assays, α-amylase inhibition, and molecular docking simulations, offering insights into its potential as an anti-diabetic agent.

Objective : This study aimed to assess the anti-diabetic properties of I. reniformis through a comprehensive analysis, including cell viability assays, in vitro α-amylase inhibitory assays, and molecular docking simulations.

Methods : The cell viability assay was performed to evaluate the cytotoxicity of I. reniformis extract on pancreatic β-cells, while the α-amylase inhibitory assay assessed its ability to inhibit the enzyme responsible for carbohydrate digestion. Molecular docking was employed to explore the potential interactions between active constituents of I. reniformis and key proteins involved in diabetes pathogenesis.

Results : I. reniformis extract demonstrated non-toxic effects on pancreatic β-cells, suggesting its safety for further investigation as a potential therapeutic agent. α-Amylase Inhibitory Assay: The extract exhibited promising inhibitory activity against α-amylase, indicating its potential to regulate carbohydrate digestion. Molecular Docking: The molecular docking analysis revealed possible interactions between active compounds in I. reniformis and crucial diabetes-related proteins, providing insights into its mode of action.

Conclusion : This study contributes to the understanding of I. reniformis as a potential anti-diabetic agent.

The non-toxicity to pancreatic β-cells, α-amylase inhibition, and molecular interactions suggest its therapeutic potential in diabetes management.