EXTRACTION AND PARTIAL CHARACTERIZATION OF ANTICOAGULANT SULPHATED CHITOSAN FROM PEN

Abstract

Introduction:

Sulfated chitosan is a modified form of chitosan, a naturally occurring biopolymer derived from chitin found in the exoskeletons of crustaceans and the pens of cephalopods.Chitosan itself has gained significant attention in various fields due to its biocompatibility, biodegradability, and versatile properties.The effectiveness of sulphation and the degree of substitution (DS) of sulphate groups will be assessed using partial characterisation techniques like elemental analysis and Fourier Transform Infrared Spectroscopy.

Materials and Method:

Chitin obtained from gladius was deacetylated by Takiguchi's technique and 40% aqueous NaOH to produce chitosan. 50 cc of DMF was used to prepare sulfated chitosan.In order to create gelatinous chitosan, SO3 was added to a 500 ml three-necked bottom flask holding 50 ml of chitosan solution in a solution of DMF and formic acid. After the reaction had been allowed to run for one to two and a half hours at the proper temperature of 40 to 60 °C, 300 cc of 95% ethanol were added to precipitate the product.Chitosan was extracted from the pen of the Sepioteuthis lessoniana using FT-IR, CHN, SEM, and XRD investigations, and it was then characterised. Purified chitosan was sulfated in N,N-dimethylformamide using chlorosulfonic acid, and the presence of the added sulfate group was confirmed via FT-IR analysis. Chitosan has high antioxidant properties, as evidenced by its capacity to scavenge DPPH, superoxide, and hydroxyl radicals as well as decrease and chelate ferrous ions.

Results:

When examined utilizing the activated partial thromboplastin time (APTT) and prothrombin time (PT), chitosan was discovered to be a powerful anticoagulant. According to the study's findings, S. lessoniana pen could be employed as an unusual source of natural  anticoagulant which can be incorporated in functional food formulations.

Conclusion:

In the current study, the crystal clear structural reveal of sulfated chitosan synthesised from Sepioteuthis lessoniana was also clearly demonstrated, demonstrating the biological potential of the sulphate group as an efficient anti-coagulant.