SYNTHESIS OF CHITOSAN SULFATE FROM MARINE SQUID WASTE AND INHIBITION OF THROMBIN FORMATION IN HUMAN BLOOD

Abstract

INTRODUCTION:

Loliolus investigatoris is a cephalopod(squid) belonging to the family Loliginidae. The pen or gladius of the squid is an internalized shell and serves as a protective barrier for the visceral organs. Gladius is often cut and discarded, but it consists of a very important homopolysaccharide, Chitin. Sulfated  Chitosan is found to possess many biological properties like antibacterial, anti microbial,non toxicity and other properties.

MATERIALS AND METHOD:

Gladius of Loliolus investigatoris were taken and Chitin was extracted from the gladius. Chitosan was sulfated to form sulfated chitosan and was sent for characteristic analysis like FTIR, SEM and XRD analysis and Anticoagulant assessment tests like aPTT and PT.

RESULTS:

From the anticoagulant assays, we can infer that sulfated chitosan possesses similar and significant anticoagulant properties as compared to heparin which is a commercial anticoagulant used in surgeries.

CONCLUSION:

Chitosan sulfate prepared from the Gladius possess similar anticoagulant property to that of heparin and hence can be used as a potential anticoagulant in clinical and surgical uses.