PROFILING OF SALIVARY ALPHA AMYLASE AMONG YOUNG ADULTS IN HUMAN SALIVA

Abstract

Introduction: Saliva is a watery fluid produced by glands present in the oral cavity. Salivary amylase is a glucose-polymer cleavage enzyme. There are two types of amylases - AMY1 and AMY2. Salivary amylase is involved in maintaining oral health by breaking food particles and preventing dental plaque formation, antimicrobial properties, Taste Perception by releasing taste compounds from food. They increase in secretion due to Stress and mainly It acts as a diagnostic marker for various conditions like diabetes, obesity, and certain types of cancer. These provide insights into overall health status and serve as a non-invasive diagnostic tool for the future.

Aim: To find human salivary Alpha amylase production and profiling among young adults.

Materials and method: Collection of salivary samples from normal healthy men and females of 20 to 30 yrs old. Extraction of functional protein from salivary samples. Salivary alpha-amylase activity and identification of amylase protein in Native PAGE.

Result: The bands visualized as transparent bands on a dark blue background are Alpha amylase. Amylase profile pattern of Female saliva was more than male. It may be due to fluctuating Hormones, such as estrogen and progesterone, Stress response and Genetic factors. In males, M2 and M3 had Alpha amylase production. Whereas M1 and M4 had very low levels. In females, All had Alpha amylase production. Comparatively F3 had more than other females.

Conclusion: We can conclude that salivary alpha production depends on Environmental factor, Genetic factor, stress response, hormone level and health status. Salivary amylase production acts as a diagnostic tool for determining health of individuals.